BT20: Biological Indicator Test for Steam Sterilisation Control (30 pcs.) - VARIOUS

Biological Indicator for Steam Sterilisation BT20

BT20 is a Bionova biological indicator manufactured for the control of sterilisation processes by favour. Ideal for vacuum assisted steam sterilisation cycles at 121-134°C and for gravity air displacement steam sterilisation cycles at 121-134°C.

It features a coloured indicator to show whether sterilisation was successful or not. If it changes from purple to yellow, it means that the sterilisation cycle has not been performed correctly as there are live Geobacillus stearothermophilus spores. If, on the other hand, it remains purple, it indicates that sterilisation was successful.

Features of the BT20 Biological Indicator:

• Biological indicator designed for the control of steam sterilisation processes.
• It has a coloured indicator to show whether sterilisation was successful or not.
• If it changes from purple to yellow, it means that live Geobacillus stearothermophilus spores are present and that the sterilisation cycle was not successful
• If it remains purple, sterilisation was successful. The final reading should be taken 24 hours after incubation.
• Ideal for vacuum assisted steam sterilisation cycles at 121-134°C and for gravity air displacement steam sterilisation cycles at 121-134°C.
• Incubate the processedbiological indicator and the indicator used as a positive control for a maximum of 24 hours at 60±2 °C.
• Content: 1 box of 30 pcs.

Technical Specifications of the BT20 Biological Indicator:

• Polypropylene random copolymer tube, 44.0 mm high, 8.5 mm outer diameter. Wall 0.5 mm thick.
• Polypropylene Ampoule 13.0 mm high, 10.5 mm external diameter. Wall 1.0 mm thick.
• Glass ampoule: 35.0 to 38.0 mm in height. External diameter: 6.5 mm. Wall 0.2- 0.3 mm thick.
• Culture medium: 0.5 - 0.7 ml, purple.
• Lid filter: medical grade paper 60 g/m2, 16.0 mm in diameter.
• Filter paper: 8.0 mm diameter and 160 g/m2.
• D-value: not less than 1.5 minutes at 121º C. Another D value at 134º C is obtained for calculating the Z value.
• Z value: not less than 6º C.

How to use the BT20 Biological Indicator:

• Step 1: Write the steriliser number (if more than one), load number and processing date on the tube label.
• Step 2: Place the biological indicator with the material to be sterilised in a suitable package according to sterilisation practices.
• Step 3: Sterilise as usual.
• Step 4: Once the sterilisation process is complete and after 5 minutes, remove the biological indicator from the package.
• Step 5: Leave to cool until it returns to room temperature.
• Step 6: Verify that the chemical indicator printed on the indicator label has changed to brown. This indicates that he was exposed to steam.
• Step 7: Break the ampoule contained in the indicator and incubate for 24 h at 60±2 ºC.
• Step 8: Observe if the colour changes from purple to yellow (unsuccessful process) or if it remains purple (successful sterilisation).